ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (1): 14-22.doi: 10.11843/j.issn.0366-6964.2014.01.003

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Mining Anti Classic Swine Fever Virus-related Genes Based on Microarray and Analyzing Their Transcriptional Expression under Different CSF-antibody Levels

WANG Xiao-shuo1YU Ying1LIU Hui-jie1XIAO Li1ZHAI Li-wei1AO Hong2*WANG Chu-duan1*   

  1. (1.College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China;2.Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China)
  • Received:2013-06-13 Online:2014-01-23 Published:2014-01-23

Abstract:

The present study was conducted to screen the anti-CSF virus-related genes and to analyze the genes expression under the different CSF-antibody levels in Landrace and Yorkshire.Agilent porcine 4×44K microarray was used to analyze and obtain the differentially expressed genes in four research groups:virus mimic PolyI:C transfected cells,DNA methytransferase inhibitor Aza-CdR transfected PK15 cells,PolyI:C and Aza-CdR transfected cells,mock cells.The differentially expressed genes were analyzed by cluster and gene ontology (GO).The results showed that significantly differentially expressed genes of myxovirus resistant (MX1) and the double-stranded-RNA-dependent protein kinase(PKR) mainly enriched in the biological process of defense response to virus.The expression levels of both genes in peripheral blood of Landrace and Yorkshire were detected under different CSF-antibody levels.The expression levels of two genes were significantly higher in both breeds with high antibody levels than that with non-immune levels (P<0.05).In addition,MX1 was highly expressed in Yorkshire peripheral blood than that in Landrace (P<0.01).Considering MX1 and PKR are important genes in interferon pathway,the results indicate that both genes can be served as potential candidate genes for defending CSFV infection.

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